Services

Working with different partners institutions, PT-OPENSCREEN offers a collection of services. 
For service or information requests, please fill the contact form and your request will be relayed to a suitable partner of the network.



Biology

From standard assays to the development of new assays and analysis of the results

Chemistry

Compound bioprofiling of small molecule libraries on the panel of cell lines and data analysis.

Consultancy & Analysis

The management and execution of projects, identification of suitable experimental approach.

Assay miniaturization

Adaptation of cell or biochemical assays to microwell plate format for screening to achieve robust and reproducible readouts with minimum intra and inter-plate variability.

CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

Compound profiling

Original small molecules or extracts from natural sources are profiled using different cellular models, including human cells from healthy and diseased-altered tissues. Assays to collect information about viability will suggest compounds with a promising therapeutic profile for follow-up experiments.

CBMA, CBA-UAC, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

Pilot screening

Screening of compound libraries of up to 5000 compounds as final job or as preparation to full screening campaigns of more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.

CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

High-Throughput screening

Screening of compound libraries more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.

i3S

Target deconvolution 

Cell and/or biochemical assays are designed and performed for target deconvolution of hits resulting from phenotypical-based screenings.

CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

Drug-target interactions

Bio-Layer Interferometry is used to perform label-free, real-time study of biomolecular interactions (drug-receptor, protein-protein interactions) for determination of binding constants and kinetics.

CEB-UM

Evaluation of drug-like properties

Evaluation of physicochemical properties (e.g. solubility, lipophilicity (LogD / LogP), pKa) that can affect biological assay results and cause incorrect structure-activity relationships. This evaluation is relevant in early drug discovery programs and necessary for ADMET studies.

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE

Hit to lead or lead optimization

For newly identified compounds with targeted biological activity (hits), optimization performed by medicinal chemistry tools is offered in order to obtain pharmacologically viable advanced candidates possessing optimized activity/potency/selectivity profiles and/or chemical novelty.

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE

Custom synthesis

Synthesis of different chemical tools such as building blocks, standards for pharmacological and analytical purposes, degradation products or impurities, human metabolites or prodrugs to improve pharmacokinetic properties, in small to medium sized libraries.

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE

Evaluation of drug or drug candidate stability

The evaluation of compound storage stability (in solution or solid state) and compound metabolic stability (namely in plasma and in hepatocytes) can be performed using chromatographic (UHPLC/HPLC) and spectrometric (NMR/MS) tools.
 
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, IMM, BioISI

Evaluation of drug or drug candidate permeability using biomimetic membrane models 

BBB, gastro-intestinal and skin permeability prediction can be attained using non-cellular (Parallel Artificial Membrane Permeability Assay) permeability assays. If needed, permeability studies using cellular assays like Caco-2 cells, can be performed.

CIQUP, CQM, CQC, iMED.Ulisboa, CQE, IMM

Quality control of compounds

The consortium has the necessary equipment to perform quality control of libraries of compounds and isolation/ purification of samples. The consortium also has equipment for chemical characterization (NMR, HPLC-MS).

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI

Construction of chemical libraries

Synthesis of small focused libraries for screening based on target or compound class, isolation of novel compounds from biological extracts (different chromatographic techniques) and characterization of new structures (NMR, HPLC-MS).

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, CIIMAR

Access to compound libraries

Chemical banking for management and storage will provide access to libraries of bioactive, target-focused or diversity-based compounds, fragments, biological extracts, to compounds produced by Portuguese chemists and, as part of the EU-OPENSCREEN, to the European Chemical Biology Library.

i3S, CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM

Access to libraries of tools for genome-wide gene perturbation

Libraries of siRNAs, microRNAs, dsRNAs (C.elegans), CRISPR (guide RNA) and yeast mutants allow the identification of disease-relevant target genes that could be further used as basis for the development of target-based chemical screens.

CNC, ICVS, BioISI, CBMA, i3S

Access to readout instruments

Access to microplate readers (absorbance, fluorescence, luminescence and related technologies) and automated widefield and confocal microscopes (high-content assays).

CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

Medicinal Chemistry projects and consulting

The consortium has experience in the management and execution of projects of preclinical Medicinal Chemistry. The consortium offers both consultancy in project management and design, synthesis of dedicated libraries and characterization of new structures (NMR, HPLC-MS).

CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI

Consultancy on experimental screening strategy

Identification of suitable experimental approach: cell or biochemical assay, chemical libraries size and composition, selection of suitable material and reagents, readout technology such as absorbance, fluorescence intensity, luminescence, high-content screening on automated fluorescence microscope.

CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CIQUP, CNC, CQM, CQUM, CQC, CQE, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S

Bioimage analysis

Automated processing of microscopy images (fluorescence or transmission) using appropriate software tools and IT infrastructure to extract quantitative phenotypic data in numerical form. Extraction of multi-parametric quantitative features at the single cell level for high content screening assays.

CEDOC, CNC, BioISI, ICVS, iMM, i3S, iBiMED

Statistical data analysis

Efficient analysis of large numerical datasets produced by spectroscopy or image-based screening using data science tools to report the phenotypic variation caused by each chemical perturbation. Will include data visualization, exploratory data analysis, quality control and normalization.

CNC, ICVS, BioISI, iMM, i3S

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