AI Website GeneratorWorking with different partners institutions, PT-OPENSCREEN offers a collection of services.
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From standard assays to the development of new assays and analysis of the results
Compound bioprofiling of small molecule libraries on the panel of cell lines and data analysis.
The management and execution of projects, identification of suitable experimental approach.
Adaptation of cell or biochemical assays to microwell plate format for screening to achieve robust and reproducible readouts with minimum intra and inter-plate variability.
CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
Original small molecules or extracts from natural sources are profiled using different cellular models, including human cells from healthy and diseased-altered tissues. Assays to collect information about viability will suggest compounds with a promising therapeutic profile for follow-up experiments.
CBMA, CBA-UAC, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
Screening of compound libraries of up to 5000 compounds as final job or as preparation to full screening campaigns of more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.
CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
Screening of compound libraries more than 100000 compounds. Includes hit confirmation and validation and may include follow-up studies.
i3S
Cell and/or biochemical assays are designed and performed for target deconvolution of hits resulting from phenotypical-based screenings.
CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
Bio-Layer Interferometry is used to perform label-free, real-time study of biomolecular interactions (drug-receptor, protein-protein interactions) for determination of binding constants and kinetics.
CEB-UM
Evaluation of physicochemical properties (e.g. solubility, lipophilicity (LogD / LogP), pKa) that can affect biological assay results and cause incorrect structure-activity relationships. This evaluation is relevant in early drug discovery programs and necessary for ADMET studies.
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE
For newly identified compounds with targeted biological activity (hits), optimization performed by medicinal chemistry tools is offered in order to obtain pharmacologically viable advanced candidates possessing optimized activity/potency/selectivity profiles and/or chemical novelty.
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE
Synthesis of different chemical tools such as building blocks, standards for pharmacological and analytical purposes, degradation products or impurities, human metabolites or prodrugs to improve pharmacokinetic properties, in small to medium sized libraries.
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE
The evaluation of compound storage stability (in solution or solid state) and compound metabolic stability (namely in plasma and in hepatocytes) can be performed using chromatographic (UHPLC/HPLC) and spectrometric (NMR/MS) tools.
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, IMM, BioISI
BBB, gastro-intestinal and skin permeability prediction can be attained using non-cellular (Parallel Artificial Membrane Permeability Assay) permeability assays. If needed, permeability studies using cellular assays like Caco-2 cells, can be performed.
CIQUP, CQM, CQC, iMED.Ulisboa, CQE, IMM
The consortium has the necessary equipment to perform quality control of libraries of compounds and isolation/ purification of samples. The consortium also has equipment for chemical characterization (NMR, HPLC-MS).
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI
Synthesis of small focused libraries for screening based on target or compound class, isolation of novel compounds from biological extracts (different chromatographic techniques) and characterization of new structures (NMR, HPLC-MS).
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, CIIMAR
Chemical banking for management and storage will provide access to libraries of bioactive, target-focused or diversity-based compounds, fragments, biological extracts, to compounds produced by Portuguese chemists and, as part of the EU-OPENSCREEN, to the European Chemical Biology Library.
i3S, CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM
Libraries of siRNAs, microRNAs, dsRNAs (C.elegans), CRISPR (guide RNA) and yeast mutants allow the identification of disease-relevant target genes that could be further used as basis for the development of target-based chemical screens.
CNC, ICVS, BioISI, CBMA, i3S
Access to microplate readers (absorbance, fluorescence, luminescence and related technologies) and automated widefield and confocal microscopes (high-content assays).
CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CNC, CQM, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
The consortium has experience in the management and execution of projects of preclinical Medicinal Chemistry. The consortium offers both consultancy in project management and design, synthesis of dedicated libraries and characterization of new structures (NMR, HPLC-MS).
CIQUP, CQM, CQUM, CQC, iMED.Ulisboa, CQE, BioISI
Identification of suitable experimental approach: cell or biochemical assay, chemical libraries size and composition, selection of suitable material and reagents, readout technology such as absorbance, fluorescence intensity, luminescence, high-content screening on automated fluorescence microscope.
CBMA, CBA-UAC, CEB-UM, CEDOC, CIEQB, CIQUP, CNC, CQM, CQUM, CQC, CQE, ToxOmics, CIIMAR, iBiMED, BioISI, ICVS, IHMT, iMED.Ulisboa, iMM, i3S
Automated processing of microscopy images (fluorescence or transmission) using appropriate software tools and IT infrastructure to extract quantitative phenotypic data in numerical form. Extraction of multi-parametric quantitative features at the single cell level for high content screening assays.
CEDOC, CNC, BioISI, ICVS, iMM, i3S, iBiMED
Efficient analysis of large numerical datasets produced by spectroscopy or image-based screening using data science tools to report the phenotypic variation caused by each chemical perturbation. Will include data visualization, exploratory data analysis, quality control and normalization.
CNC, ICVS, BioISI, iMM, i3S
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